rabbit anti human il 22r1 polyclonal antibodies  (ProSci Incorporated)

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-22 in rheumatoid arthritis: potential role as a proinflammatory cytokine.

doi: 10.1002/art.20965

Figure Lengend Snippet: Figure 4. Interleukin-22 receptor 1 (IL-22R1) mRNA expression in rheumatoid arthritis (RA) synovial tissues. Reverse transcription– polymerase chain reaction for IL-22R1 or -actin was performed using mRNA extracted from the synovial tissues of 4 RA patients (RA1– RA4). Hep-G2 cells were used as a positive control.

Article Snippet: Rabbit anti-human IL-22R1 polyclonal antibodies were from Prosci Incorporated (Poway, CA).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Positive Control

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-22 in rheumatoid arthritis: potential role as a proinflammatory cytokine.

doi: 10.1002/art.20965

Figure Lengend Snippet: Figure 5. Immunohistologic localization of IL-22R1 in synovial tissue from either patients with RA (A and C–J) or patients with osteoar- thritis (B). Dual-labeling immunofluorescence staining of RA synovial tissues (C–J) was done with anti–IL-22R1 (green) (C and G), anti- vimentin (red) (D), and anti-CD68 (red) (H). Staining of nuclei was done with 4,6-diamidino-2-phenylindole (blue) (E and I). Merged images are shown in F (merger of C, D, and E) and J (merger of G, H, and I). See Figure 4 for definitions. (Counterstained with hematoxylin; original magnification 200.)

Article Snippet: Rabbit anti-human IL-22R1 polyclonal antibodies were from Prosci Incorporated (Poway, CA).

Techniques: Labeling, Immunofluorescence, Staining

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-22 in rheumatoid arthritis: potential role as a proinflammatory cytokine.

doi: 10.1002/art.20965

Figure Lengend Snippet: Figure 6. Expression of IL-22 (A) and IL-22R1 (B and C) in synovial fibroblasts established from RA tissues (RASF) obtained from 4 RA patients (RA1–RA4). Reverse transcription–polymerase chain reac- tion for IL-22 (A), IL-22R1 (B), or -actin was performed using mRNA extracted from RASF. Western blotting was performed using specific antibodies against IL-22R1 (C). Peripheral blood mono- nuclear cells from normal donors, stimulated with phytohemagglutinin (PC) or without phytohemagglutinin (NC), were used as positive and negative controls, respectively (A). Hep-G2 was used as a positive control for IL-22R1 (B and C). See Figure 4 for other definitions.

Article Snippet: Rabbit anti-human IL-22R1 polyclonal antibodies were from Prosci Incorporated (Poway, CA).

Techniques: Expressing, Reverse Transcription, Western Blot, Positive Control